Marque Bretagne


The solution for graft preservation

The annual number of transplants is constantly increasing in France, from 4,580 in 2009 to 6,105 in 2017.However, this still falls well short of demand. In 2017, 23,828 patients were waiting for organs1. 400 to 500 people on the transplant waiting list dies due to the lack of available organs.
Faced with this shortage, regulatory and health authorities have authorized the use of organs from older and older donors and from donors who suffered cardiac death. Organs from such donors are very sensitive to the ischemia-reperfusion cycle.

In the USA, more than 123,000 people are waiting for transplants2. Yet, one in eight organs collected is not transplanted because there is too much time between collection and transplantation.

During a transplant of “solid” organs, the organ removed is experiencing a sudden stop of blood supply and therefore the oxygen necessary for its survival (i.e. ischemia). It is then usually placed in a 4°C preservation solution to reduce its metabolism (Figure 1).
Ischemia/reperfusion can lead to lesions due to the lack of oxygenation of the graft, and these play an important role in the risk of primary rejection of the graft by the recipient. Ischemia/reperfusion can lead to lesions due to the lack of oxygenation of the graft, and these play an important role in the risk of primary rejection of the graft by the recipient.

Figure 1: The ischemia-reperfusion cycle is one of the major problems of transplantation

The challenge is therefore major to provide solutions to limit these lesions and thus improve the quality of the grafts.

In this context, the HEMO2life® medical device developed by Hemarina represents a breakthrough innovation in the field of organ preservation. HEMO2life® is an additive to organ preservation solutions, allowing the graft to be oxygenated physiologically and thus considerably reducing the risk of graft rejection and extending the storage time of the grafts.

HEMO2life® has already been tested on several pre-clinical models, and has been the subject of a clinical study in kidney transplantation (OxyOp) led by Prof. Yannick Le Meur (CHRU Brest) and Prof. Benoît Barrou (APHP, Pitié-Salpêtrière Paris), that included 60 patients, at 6 primary French transplantation centres with remarkable results.

In 2018, HEMO2life® was also used during a patient’s second total face graft, performed by Professor Lantiéri (Georges Pompidou European Hospital). The immediate benefit of the widespread use of this technology would be twofold: to provide transplant surgeons with organs in excellent physiological conditions and provide better preservation conditions for the graft while it is in transit. This would increase the time the organ could survive between the time of collection and transplantation to the recipient,
which preliminary results of the OxyOp clinical study showed that preserving the graft with HEMO2life® allows a better recovery of the transplanted organ’s function and would thus increase the success rate of the grafts performed.

Today, harvested organs can only survive a few hours post removal, after which time they lose their metabolic functions and intrinsic quality 3. Hemarina’s, HEMO2life® allows longer possible preservation time of the harvested organs. 3 HEMO2life® can be easily integrated into current hospital practices and could greatly facilitate the organisation of the transplant by giving medical staff more time to prepare the transplant. Hemarina submitted a “CE” marking application file in 2018 to a notified body.

1. Biomedicine Agency, press release, March 2018
2. Number of candidates for transplantation in the United States in 2015, Statista, 2019
3. From 3 hours for a heart, to 24 hours for a Kidney, Biomedicine Agency, Organ Donation,

HEMO2life® : Related Publications

Asong-Fontem, N., Panisello-Rosello, A., Lopez, A., Imai, K., Zal, F., Delpy, E., Rosello-Catafau, J., Adam, R. A Novel Oxygen Carrier (M101) Attenuates Ischemia-Reperfusion Injuries during Static Cold Storage in Steatotic Livers Int. J. Mol. Sci. - 2021

Ali, A., Watanabe, Y., Galasso, M., Watanabe, T., Chen, M., Fan, E., Brochard, L., Ramadan, K., Ribeiro, RVP., Stansfield, W., Gokhale, H., Gazzalle, A., Waddell, T., Liu, M., Keshavjee, S., Cypel, M. An extracellular oxygen carrier during prolonged pulmonary preservation improves post-transplant lung function J Heart Lung Transplant. - 2020

Le Meur, Y., Morelon, E., Essig, M., Thierry, A., Büchler, M., Drouin, S., Deruelle, C., Badet, L., Pesteil, F., Delpech, P-O., Boutin, J-M., Renard, F., Barrou, B. First-in-human use of a marine oxygen carrier for organ preservation: a safety and proof-of-principle study. Am J Transplant. - 2020

Alix, P., Val-Laillet, D., Turlin, B., Ben Mosbah, I., Burel, A., Bobillier, E., Bendavid, C., Delpy, E., Zal, F., Corlu, A., Boudjema, K. Adding the oxygen carrier M101 to a cold-storage solution could be an alternative to HOPE for liver graft preservation. JHEP Rep. - 2020

Lemaire, F., Sigrist, S., Delpy, E., Cherfan, J., Peronet, C., Zal, F., Bouzakri, K., Pinget, M., Maillard, E. Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas. Journal of Cellular and Molecular Medicine. - 2019

Kaminski, J., Hannaert, P., Kasil,A., Thuillier, R., Leize, E., Delpy, E., Steichen, C., Goujon, J.-M., Zal, F., Hauet, T. Efficacy of the natural oxygen transporter HEMO2life® in cold preservation in a preclinical porcine model of donation after cardiac death. Transplant International, official journal of the European Society for Organ Transplantation. - 2019

Thuillier, R., Delpy, E., Matillon, X., Kaminski J., Kasil, A., Soussi, D., Danion, J., Sauvageon, Y., Rod, X., Donatini, G., Barrou, B., Badet, L., Zal, F., Hauet, T. Preventing acute kidney injury during transplantation: the application of novel oxygen carriers. Expert Opinion on Investigational Drugs, 28(7):643-657. - 2019

Kasil, A., Giraud, S., Couturier, P., Amiri, A., Danion, J., Donatini, G., Matillon, X., Hauet, T., Badet, L. Individual and combined impact of oxygen and oxygen transporter supplementation during kidney machine preservation in a porcine preclinical kidney transplantation model. International Journal of Molecular Sciences, 20(8). - 2019

Glorion, M., Polard, V., Favereau, F., Hauet, T., Zal, F., Fadel, E., Sage, E. Prevention of ischemia-reperfusion lung injury during static cold preservation by supplementation of standard preservation solution with HEMO2life® in pig lung transplantation model. Artificial Cells, Nanomedicine, and Biotechnology, 46(8):1773-1780. - 2018

Ali, A., Watanabe, Y., Galasso, M., Stansfield, W., Watanabe, T., Ramandan, K., Chen, M., Ribeiro, R.P., Gokhale, H., Gazzalle, A., Waddell, T., Liu, M., Keshavjee, S., Cypel, M. Two-Day Lung Preservation Followed by Lung Transplantation in a Large Animal Model Using Novel Extracellular Oxygen Carrier. The Journal of Heart and Lung Transplantation, - 2018

Lemaire, F., Bietiger, W., Peronet, C., Langlois, A., Mura, C., Bouzakri, K., Sigrist, S., Polard, V., Zal, F., Pinget, M., and Maillard, E. Effect of HEMO2life®, a marine oxygen carrier, for pancreas preservation during cold ischemia. Poster presented during 8th international EPITA Symposium, Innsbruck, Austria. - 2018

Polard, V., Alix, P. Evaluation of a specific oxygen carrier (M101®) added to pig liver cold storage solutions to improve post-transplant graft function. Presented at the XVI ISBS, held November 13-15, 2017 in Montréal, Canada. - 2017

Teh, E. S., Zal, F., Polard, V., Menasché, P., Chambers, D.J. HEMO2life® as a protective additive to Celsior solution for static storage of donor hearts prior to transplantation. Artificial Cells, Nanomedicine, and Biotechnology, 45(4):717-722. - 2017

Mallet, V., Dutheil, D., Polard, V., Rousselot, M., Leize, E., Hauet, T., Goujon, J.-M., Zal, F. Dose-Ranging study of the performance of the natural oxygen transporter HEMO2life® in organ preservation. Artificials Organs, 38(8):691-701. - 2014

Zal, F., Rousselot, M. Extracellular Hemoglobins from Annelids, and their Potential Use in Biotechnology Outstanding Marine Molecules, Wiley‐VCH Verlag GmbH & Co. KGaA, - 2014

Thuillier, R., Dutheil, D., Trieu, M.T.N., Mallet, V., Allain, G., Rousselot, M., Denizot, M., Goujon J.-M., Zal, F., Hauet, T. Supplementation with a new therapeutic oxygen carrier reduces chronic fibrosis and organ dysfunction in kidney static preservation. American Journal of Transplantation, 11(9):1845-1860. - 2011

Le Meur, Y., Delpy, E., Renard, F., Hauet, T., Badet, L., Rerolle, JP., Thierry, A., Büchler, M., Zal, F., Barrou, B. HEMO2life® improves renal function independent of cold ischemia time in kidney recipients: A comparison with a large multicenter prospective cohort study Artificial Organs. - 2021

Latest press releases

Hemarina and Delpharm announce the signing of a partnership for the manufacture of HEMO2life®
November 29, 2018


Brest University Hospital and HEMARINA present the new positive advances of the first clinical trial of HEMO2life® at the American Transplant Congress in Seattle,
June 6, 2018


Hemarina and the University Health Network of Toronto announce positive preclinical proof of concept results for the HEMO2life® oxygen transporter in the preservation of lung transplants before transplantation
April 12, 2018


Brest University Hospital and HEMARINA announce positive results from the first clinical trial of HEMO2life® in kidney conservation before transplantation in humans.
November 14, 2017